The white/greyish basidiospores were generally sausage-shaped (allantoid) ranging in length (18.47☐.73μm) and width (7.49☐.3μm) closely to reported range. The dimensions of the basidia (70.86±4.27 μm long 9.54± 0.60 μm wide) closely agreed with the range reported for A. Anatomical and morphometric studies showed that the fruiting body had smooth hymenium with a layer of basidia which were cylindrical or club-shaped. The general morphological features were akin to that of Auricularia auricula-judae. The basidiomata were either solitary, densely rosette with caestipose habit either astipitate or with vestigial stipe. The fruiting body was gelatinous with the shape of a human ear. A mushroom of recognised health benefits was seen for the first time on a pencil cactus (Euphorbia turicalli L.) in the Greater Accra Region (Adentan Municipal Assembly) in September- October 2021. Medicinal mushrooms are part of total health delivery system of a nation. In general, the findings affirmed that PLS is efficient on wound closure in alloxan-induced diabetic rats. Furthermore, the histological assessment of the PLS-treated group demonstrated complete reepithelialized wounds by accelerating collagen synthesis. Indeed, its application significantly increased wound contraction percentage ( 98 ± 1.11 % ) after 14 days of experiment. These results strongly support the beneficial effects of the PLS to accelerate wound healing in diabetic rats. This novel polysaccharide had an average molecular weight of 98.51 kDa and displayed potential antioxidant activities determined through three different assays: scavenging activity against 2,2 ′ -azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, and reducing power. Results obtained by 1H NMR indicated that PLS consisted of three monosaccharide residues with α and β anomers. According to HPLC, the crude PLS is a heteropolysaccharide composed of glucose, xylose, and galactose. The FT-IR and UV spectra showed the characteristic band of polysaccharides. The PLS was structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, UV spectrum, high performance liquid chromatography (HPLC), gas chromatography (GC), nuclear resonance magnetic (NMR), and high-pressure gel filtration chromatography. This polysaccharide presented a lighter natural color, whose luminosity ( L ∗ ), red-green intensity ( a ∗ ), and blue-yellow intensity ( b ∗ ) were recorded at 63.26, 5.87, and 27.28, respectively.
The present study undertakes the extraction of a novel polysaccharide from Lepidium sativum (PLS) and the determination of its physicochemical composition and antioxidant properties, as well as its potential wound healing activity in alloxan-induced diabetic rats. Overall, our results indicate that the wound-healing activities of AAP can be applied in an AAP-based product for wound management.
#Right inferioir post auricula skin skin
Specifically, the AAP significantly accelerated the wound closure in a mice skin wound-healing model on day 9 (2.5%AAP, p = 0.031 vs. AAP significantly promoted both fibroblast and keratinocyte proliferation, migration, and invasion, along with augmentation of the wound-healing process by increasing collagen synthesis and decreasing E-cadherin expression (All p < 0.05). AAP displayed antioxidant activity in vitro and was able to abort UVB-induced intracellular ROS production in human fibroblasts in cellulo. The main sugar composition of AAP includes mannose, galactose, and glucose. AAP contained high amounts of polysaccharides (349.83 ± 5.00 mg/g extract) with a molecular weight of 158 kDa. In this study, we have reported on the mechanisms that promote the wound-healing effects of a water-soluble polysaccharide-rich extract obtained from A. Auricularia auricula-judae, a nutrient-rich mushroom used in traditional medicine, is a macrofungi that exhibits various biological properties.
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